|
KMID : 0613820120220060823
|
|
Journal of Life Science
2012 Volume.22 No. 6 p.823 ~ p.827
|
|
|
Effect of Tryptic Soy Broth (TSB) and Luria-Bertani (LB) Medium on Production of Subtilisin CP-1 from Bacillus sp. CP-1 and Characterization of Subtilisin CP-1
|
|
Park Chang-Su
|
|
|
Abstract
|
|
|
|
A bacterial strain producing a fibrinolytic enzyme, subtilisin CP-1, was isolated from Doen-Jang, a Korean traditional fermentation food. Based on the analysis of gene sequence of 16S rRNA and biochemical analysis, the strain was identified as Bacillus sp. and named as Bacillus sp. CP-1. To investigate the effect of the medium on the production of fibrinolytic enzyme from Bacillus sp. CP-1, two commercial bacterial culture media, tryptic soy broth (TSB) and Luria-Bertani (LB), were applied to the cultivation of Bacillus sp. CP-1. The strain secreted only one proteolytic enzyme (subtilisin CP-1) in the culture broth. The molecular weight of subtilisin CP-1 was estimated to be 28 kDa. Subtilisin CP-1 was optimally active at pH 9.0 and 45℃, and exhibited high specificity for Meo-Suc-Arg-Pro-Tyr-pNA (S-2586), a synthetic chromogenic substrate for chymotrypsin. The first eight amino acid residues of the N-terminal sequence of the enzyme are AQSVPYGI; this sequence is identical to that of subtilisin NAT and E.
|
|
|
KEYWORD
|
|
|
Bacillus, culture medium, extracellular protease, fibrinolytic enzyme, subtilisin
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|